河北环境工程学院学报

为开发用于铬污染土壤修复的功能菌肥,以铬污染土壤为研究对象,通过富集培养、梯度驯化等方法分离筛选高效耐铬菌株,并优化菌株发酵条件,制备降铬功能菌肥。实验结果表明,菌株S1在pH为8.0、盐度为2%的条件下生长最佳,对铬(Ⅵ)的耐受质量浓度超过400 mg/L。进一步确定菌肥最佳制备配比为每100 g发酵牛粪添加20 mL S1菌液;在铬污染土壤中每100 g土壤添加4 g菌肥施用,可实现约85%的铬降解率。

To develop a functional bacterial fertilizer for the remediation of chromium-contaminated soil,this study took chromium-contaminated soil as the research object.Through methods such as enrichment culture and gradient domestication,highly efficient chromium-resistant strains were isolated and screened,and the fermentation conditions of the strains were optimized to prepare a chromium-reducing functional bacterial fertilizer.The experimental results showed that the screened chromium-resistant strain S1 grew best under the culture conditions of pH 8.0 and 2% salinity,with a tolerance concentration to chromium exceeding 400(Ⅵ) mg/L.Furthermore,the optimal preparation ratio for the bacterial fertilizer was determined as adding 20 mL of S1 bacterial solution to 100 g of fermented cow manure.When applied to contaminated soil at a rate of 4 g of bacterial fertilizer per 100 g of soil,approximately 85% of the chromium contamination could be degraded.This study provides a theoretical basis and technical support for the bioremediation of chromium pollution and the development of functional bacterial fertilizers.